Skip to main content
. 1999 Sep;10(9):2905–2918. doi: 10.1091/mbc.10.9.2905

Figure 8.

Figure 8

Interactions among top2, rad18 and brc1. (A) top2-191 is synthetically lethal with rad18-74 and rad18-X. Strains were plated on YES and incubated at the indicated temperatures for either 4 d (25°C) or 3 d (30 and 36°C). Strains: (1) wild type; (2) rad18-74; (3) rad18-74 top2-191; (4) rad18-X top2-191; (5) rad18-X; 6, top2-191. (B) top2-191 is synthetically lethal with brc1::ura4. Strains were plated on YES and treated as above. Strains: (1) wild type; (2) brc1::ura4; (3) brc1::ura4 top2-191; (4) top2-191. (C–J) DAPI and phase-contrast images of cells grown to midlogarithmic phase at 25°C and then shifted to 30°C for 4 h. Strains: (C) wild type; (D) rad18-X; (E) rad18-74; (F) top2-191; (G) brc1::ura4; (H) top2-191 rad18-X; (I) top2-191 rad18-74; (J) top2-191 brc1::ura4. Bar, 10 μm.