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. 2008 Oct 2;3(10):e3321. doi: 10.1371/journal.pone.0003321

Figure 1. CSF-circulating DCs infiltrate the periventricular parenchyma.

Figure 1

Fluorescent microspheres or DCs labeled with the cytoplasmic fluorescent marker CFSE were injected into the left lateral ventricle of EAE rats (n = 32) at the clinical peak of disease (day 12 post-immunization). EAE rats injected with fluorescent microspheres (n = 14) or CFSE-labeled DCs (n = 18) were then sacrificed on day 1 post-injection (n = 16) or 8 post-injection (n = 16). An immunohistological analysis of brains was then performed using antibodies directed against CD11b/CD11c (OX42) or MHC class II molecules. A–C: OX42+ cells (green) harboring intracytoplasmic fluorescent microspheres (red) are observed in the lumen of the injected lateral ventricle, on day 1 post-injection. D: Microspheres+/OX42+ cells localize in the periventricular parenchyma of the injected ventricle, on day 1 post-injection. Insert (solid square) shows a high magnification view of microspheres+/OX42+ cells infiltrating a periventricular inflammatory lesion (dashed square). E: On day 1 post-injection, MHC class II+ cells (green) harboring intracytoplasmic fluorescent microspheres (red) are detectable in the lumen of the third ventricle (dashed square) and in a large periventricular area infiltrated with MHC class II+ cells. Insert (solid square) shows a high magnification view of microspheres+/MHC class II+ cells that localize in the lumen of the third ventricle (dashed square). Arrows indicate microspheres+/MHC class II+ cells in the periventricular parenchyma. F–H: CFSE+ DCs (green) that express OX42 (red) are detected in the lumen of the injected lateral ventricle, on day 1 post-injection. I: A periventricular area bordering the injected lateral ventricle is infiltrated by OX42+ cells and contains OX42+/CFSE+ DCs, on day 1 post-injection. J: On day 8 post-injection, CFSE+ DCs are observed in a periventricular inflammatory lesion, adjacent to the injected lateral ventricle. LV: lateral ventricle, V3: third ventricle. Scale bars: 100 µm (B–J), 50 µm (A–C, E–I), 10 µm (inserts in panel D and E).