Table 1.
Binding parameters characterizing the interaction of MIP-1α variants with CCR5
| Variant | Ki, nM |
|---|---|
| MIP-1α | 5.28 ± 0.001 |
| T8A | 18.66 ± 0.15* |
| A9Q | 20.27 ± 0.03** |
| F12A | 16.59 ± 0.07** |
| Y14A | 28.78 ± 0.11** |
| T15A | 5.47 ± 0.1 |
| R17A | 16.57 ± 0.17* |
| Q18A | 7.68 ± 0.04 |
| P20A | 25.3 ± 0.06** |
| Q21A | 8.43 ± 0.05 |
| N22A | 6.183 ± 0.13 |
| A25Q | 14.52 ± 0.17* |
| K44A | 16.81 ± 0.09* |
| R45A | 58.26 ± 0.03** |
| R47A | 52.1 ± 0.14** |
| Q48E | 6.12 ± 0.06 |
| R17E | 24.2 ± 0.08* |
| Q18E | 12.09 ± 0.1**** |
| N22E | 9.57 ± 0.09**** |
The Ki value for each mutant was obtained in a competitive binding assay by using a CCR5-expressing CHO cell line and europium-labeled MIP-1α as a tracer. The values represent the mean ± SEM resulting from at least two independent experiments. The statistical significance as compared with that of wild-type MIP-1α was calculated with Student's t test.
*, P < 0.01;
**, P < 0.001;
***, P < 0.05.