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2-DE comparison between T. cruzi motile life forms using alkaline "two-in-one gel". IPG strips with epimastigote (E) and trypomastigote (T) samples were applied side-by-side on 12% T SDS-PAGE (see Material and Methods). A: "two-in-one gel" of 6–8.5 pH range. Spot 29 is shown in an inset (B) of a separate experiment. C: "two-in-one gel" of 8.5–11 pH range. Gels were silver stained.
