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. 1999 Sep;10(9):2919–2931. doi: 10.1091/mbc.10.9.2919

Figure 1.

Figure 1

(A) Western blot analysis of the expression of markers of synaptic-like microvesicles, LDCGs, and plasma membrane proteins related to the apparatus for regulated secretion. Protein (100 μg) from either wild-type PC12 (WT) or trk-PC12 (Trk+) cells lysed under control conditions or after treatment with NGF (50 ng/ml) for 24 h was loaded into each lane. Although all markers analyzed are present in wild-type PC12 cells, their levels are below the threshold of detection in trk-PC12 cells, both before and after NGF treatment. p38, p38/synaptophysin; StgI, synaptotagmin I; SynII, synapsin II; CgB, chromogranin B; SgII, secretogranin II. (B) Northern blot analysis of representative markers of the secretory apparatus. Total RNA (20 μg) extracted from wild-type PC12 or trk-PC12 cells under control conditions or after treatment with NGF for 24 h was loaded into each lane and analyzed using probes specific for p38/synaptophysin (p38) or secretogranin II (SgII) mRNAs. The mRNAs coding for either protein product appear to be barely visible or undetectable in trk-PC12 cells. Northern blot signals were compared with those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA.