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Morphological analysis of the effect of BotNC on NGF-dependent neurite outgrowth in trk-PC12 cells. Cells were observed and photographed by phase-contrast microscopy under control conditions (A) or after 48 h of NGF treatment either in the absence (B) or in the presence (C) of BotNC (0.5 nM). The toxin is apparently unable to prevent NGF-dependent neurite extension in trk-PC12 cells. Bar, 70 μm.
