Figure 7.

Analysis of the effect of antisense and sense oligonucleotides to GDI1 on NGF-dependent neurite outgrowth in trk-PC12 cells. (A) The length of neurites was measured on digitized images of trk-PC12 cells photographed by phase-contrast microscopy after 24 and 48 h of treatment with NGF in the presence of either 50 μM antisense oligonucleotides to GDI1 message (AS) or the corresponding sense oligonucleotides (S). Bars represent means ± SE. A.U., arbitrary units. (B) Western blot analysis of the corresponding samples probed with an anti-GDI α antibody. Note the selective reduction in the levels of the α isoform and the compensatory increase in the levels of the β isoform in the antisense-treated cells.