Table 1.
Western blot and immunofluorescence analysis of the expression of components of the machinery for neurosecretion in wild-type PC12 and trk-PC12 cells
| Antigen | WT/UN
|
WT/NGF
|
Trk+/UN
|
Trk+/NGF
|
||||
|---|---|---|---|---|---|---|---|---|
| WB | IF | WB | IF | WB | IF | WB | IF | |
| p38 | ++++ | ++++ | +++ | +++ | − | − | − | − |
| SynIIa | − | − | − | − | − | − | − | − |
| SynIIb | ++ | +++ | ++ | + | − | − | − | − |
| StgI | +++ | n.d. | +++ | n.d. | − | n.d. | − | n.d. |
| VAMP-1 | + | ++ | +/− | + | − | − | − | − |
| VAMP-2 | ++ | +++ | ++ | ++ | +/− | + | − | +/− |
| Syntaxin-1 | ++ | n.d. | ++ | n.d. | − | n.d. | − | n.d. |
| SNAP-25 | +++ | +++ | +++ | +++ | − | − | − | − |
| Rab3a | ++ | n.d. | + | n.d. | − | n.d. | − | n.d. |
| CgB | ++ | ++++ | + | +++ | − | − | − | − |
| SgII | ++++ | ++++ | ++++ | ++++ | − | − | − | − |
| TyrH | +++ | n.d. | +++ | n.d. | +/− | n.d. | +/− | n.d. |
| ChAT | ++ | n.d. | ++ | n.d. | +/− | n.d. | + | n.d. |
WT/UN, wild-type PC12/untreated; WT/NGF, wild-type PC12/NGF 24 h; Trk+/UN, trk-PC12/untreated; Trk+/NGF, trk-PC12/NGF 24 h; n.d., not determined; p38, p38/synaptophysin; SynIIa and SynIIb, synapsin IIa and IIb; StgI, synaptotagmin I; VAMP-1 and VAMP-2, vesicle-associated membrane protein-1 and -2; SNAP-25, synaptosome-associated protein of 25 kDa; CgB, chromogranin B; SgII, secretogranin II; TyrH, tyrosine hydroxylase; ChAT, choline acetyltransferase.