TABLE 1.
Relative BiP and PDI overexpression levels used to produce the corresponding relative SscFv levels 50 h into the in silico experiments (columns 2–14), which were normalized to provide the scaled relative SscFv level data found in Fig. 2 C
| Model | 1 | 2 | 3 | 3* | 4 | 4* | 5 | 5* | 6 | 6* | 7 | 8 | Detailed | Experiment |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Relative overexpression level | ||||||||||||||
| BiP | 2 | 1.5 | 1.5 | 40 | 1.5 | 40 | 1.5 | 20 | 2 | 40 | 3 | 100 | 2 | 3–12 |
| PDI | 2 | 2 | 10 | 40 | 10 | 40 | 10 | 80 | 10 | 40 | 20 | 15 | 4 | 6–8 |
| Relative scFv level | ||||||||||||||
| Nominal | 1.8 | 1.1 | 1.5 | 1.4 | 0.97 | 0.90 | 1.4 | 1.4 | 0.97 | 0.90 | 1.5 | 0.87 | 0.99 | 1.0 |
| O.BiP | 3.7 | 1.7 | 2.2 | 2.3 | 1.5 | 1.6 | 2.2 | 2.2 | 2.0 | 1.7 | 2.1 | 1.3 | 1.5 | 1.2 ± 0.1 |
| O.PDI | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.9 | 1.6 ± 0.4 |
| O.BiP + PDI | 3.7 | 2.8 | 2.8 | 3.0 | 2.8 | 3.0 | 2.8 | 2.9 | 3.7 | 3.0 | 3.1 | 2.9 | 2.7 | 3.4 ± 0.2 |
Experimental values of the BiP and PDI overexpression levels and the relative SscFv levels to which the data in Fig. 2 C were normalized are also included in the final column for comparison. Key: O., overexpressed; O.BiP, overexpressed BiP; O.PDI, overexpressed PDI; O.BiP+PDI, overexpressed BiP and PDI; Nominal, no overexpression.