TABLE 3.

Parameters definitions, values, and references

Developing models parameter	Detailed model parameters	Definition	Value	Units	Reference(s)
k1	N/A	Effective second-order rate constant for scFv translation and translocation into the ER by bound BiP.	1.2 × 10−8		(32,84,86)
k1b	N/A	Effective second-order rate constant for scFv translation and BiP-catalyzed translocation into the ER.	1.2 × 10−8		(32,84,86)
k1bit	N/A	Effective first-order rate constant for scFv translation and BiP-independent translocation into the ER.	4.0 × 10−3		(32,84,86)
k2	k61	PDI-UscFv binding rate.	2.3 × 10−7		(71–73)
k2b	N/A	PDI-catalyzed UscFv folding rate.	1.6 × 10−7		(36,67,68,71–73)
kr2	kr61	PDI-UscFv release rate.	6.0 × 10−4		(71–73)
k3	k63	BiP·UscFv folding rate.	7.0 × 10−3		(36,67,68)
kd	k3d	Cytoplasmic scFv mRNA degradation rate.	2.6 × 10−3		(85)
km3	km63	BiP·UscFv misfolding rate.	3.9 × 10−2		(36,67,68)
k4	k65	BiP·PDI·UscFv folding rate.	3.4		(36,67,68)
km4	km65	BiP·PDI·UscFv misfolding rate.	3.9 × 10−2		(36,67,68)
k5	k66	PDI·UscFv folding rate.	8.5 × 10−2		(36,67,68)
km5	km66	PDI·UscFv misfolding rate.	3.4 × 10−1		(36,67,68)
k6	k60	BiP-UscFv binding rate.	1.2 × 10−8		(86)
kr6	kr60	BiP-UscFv release rate.	1.0 × 10−1		(86,87)
k7	k64	UscFv folding rate.	7.0 × 10−3		(36,67,68)
km7	km64	UscFv misfolding rate.	3.4 × 10−1		(36,67,68)
N/A	k55	scFv mRNA nuclear translocation rate.	1.8		(74–80)
N/A	k56	scFv translation rate.	6.2 × 10−2		(81,82)
N/A	k58	UscFv trafficking rate to the translocon.	2.2 × 10−9		(83)
N/A	k59	UscFv posttranslational translocation rate.	9.0 × 10−1		(84)
N/A	k69	BiP-unfolded protein binding rate.	8.8 × 10−2		(S. Hildebrandt, D. Raden, A. S. Robinson, and F. J. Doyle III, unpublished)
N/A	kr69	BiP-unfolded protein release rate.	1.0 × 10−1		(S. Hildebrandt, D. Raden, A. S. Robinson, and F. J. Doyle III, unpublished)
N/A	k70	PDI-unfolded protein binding rate.	2.3 × 10−7		(71–73)
N/A	kr70	PDI-unfolded protein release rate.	6.0 × 10−4		(71–73)
N/A	k76	PDI-unfolded protein binding rate as a chaperone.	2.3 × 10−7		(71–73)
N/A	kr76	PDI-unfolded protein release rate as a chaperone.	3.2 × 10−1		(41,71–73)
N/A	k57	Cytoplasmic UscFv degradation rate.	4.0 × 10−4		(69)