TABLE 2.
Interdigitated one-start helix fibers with low nucleosome tilt (ID20)
| NRL (bp) | γ (°) | FSC | PCA ratio | Sedimentation coefficient (S) | Diffusion coefficient (× 10−8 cm2s−1) | Fiber mass density (Nucleosomes/11 nm fiber) | Fiber diameter (nm) | Persistence length (nm) |
|---|---|---|---|---|---|---|---|---|
| 187 | −60 | 0.92 ± 0.08 | 3.5 ± 1.4 | 189 ± 19 | 5.2 ± 0.5 | 4.2 ± 0.3 | 34.9 ± 0.4 | 21 ± 17 |
| 197 | −37 | 0.77 ± 0.09 | 2.7 ± 1.0 | 186 ± 18 | 4.9 ± 0.5 | — | — | — |
| 207 | −26 | 0.59 ± 0.06 | 1.8 ± 0.4 | 204 ± 12 | 5.2 ± 0.3 | — | — | — |
| 217 | −20 | 0.55 ± 0.09 | 1.9 ± 0.6 | 168 ± 10 | 4.2 ± 0.3 | — | — | — |
| 227 | −17 | 0.60 ± 0.11 | 2.4 ± 0.8 | 147 ± 13 | 3.5 ± 0.3 | — | — | — |
| 237 | −14 | 0.70 ± 0.11 | 3.0 ± 1.0 | 123 ± 12 | 2.9 ± 0.3 | — | — | — |
Simulations were conducted with the following parameters: 100 nucleosomes, 5.5 nucleosomes per turn, α = −114°, β = 0°, δ = 20°, φ = 0°, ɛ = 0°, c = 6.0 nm, d = 3.7 nm, Emax = 6.1 kT.