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. 2008 Jul 18;95(8):4068–4076. doi: 10.1529/biophysj.108.135079

TABLE 2.

Fluorescence lifetime analysis of DASPMI-stained living XTH2 cells

Condition a1 τ1 (ns) a2 τ2 (ns) a3 τ3 (ns) τ〉 (ns) f1(%) f2(%) f3(%)
Punctated 0.82 ± 0.09 0.065 ± 0.023 0.16 ± 0.08 0.626 ± 0.082 0.02 ± 0.01 2.802 ± 0.410 0.216 ± 0.065 24 ± 3 44 ± 5 32 ± 4
Elongated 0.72 ± 0.04 0.183 ± 0.041 0.24 ± 0.03 0.739 ± 0.114 0.03 ± 0.01 2.610 ± 0.241 0.396 ± 0.068 34 ± 5 45 ± 3 21 ± 4
Nucleus 0.67 ± 0.06 0.190 ± 0.071 0.25 ± 0.03 0.963 ± 0.282 0.08 ± 0.03 3.020 ± 0.500 0.603 ± 0.170 21 ± 3 39 ± 5 40 ± 8
Nucleoli 0.64 ± 0.07 0.225 ± 0.079 0.26 ± 0.05 1.257 ± 0.243 0.10 ± 0.02 3.576 ± 0.352 0.816 ± 0.084 18 ± 6 39 ± 1 43 ± 5
Cytosol 0.76 ± 0.16 0.222 ± 0.134 0.19 ± 0.12 1.073 ± 0.718 0.06 ± 0.04 3.258 ± 0.745 0.500 ± 0.066 30 ± 23 36 ± 3 34 ± 19

High SDs result from biological variations occurring from measurements on a single-cell or single-mitochondrion level, and not from instrumental inaccuracy.