TABLE 3.

Influence of lifetime parameters of DASPMI in living XTH2 cells under conditions manipulating membrane potential

Condition	a1	τ1 (ns)	a2	τ2 (ns)	a3	τ3 (ns)	〈τ〉 (ns)	f1 (%)	f2 (%)	f3 (%)
Control	0.74 ± 0.05	0.159 ± 0.051	0.23 ± 0.04	0.701 ± 0.115	0.03 ± 0.01	2.540 ± 0.227	0.357 ± 0.078	33 ± 6	45 ± 4	22 ± 5
Nigericin	0.91 ± 0.06	0.038 ± 0.028	0.07 ± 0.05	0.699 ± 0.063	0.01 ± 0.01	2.868 ± 0.259	0.118 ± 0.064	27 ± 9	41 ± 3	32 ± 8
ATP	0.86 ± 0.06	0.093 ± 0.058	0.13 ± 0.05	0.762 ± 0.104	0.02 ± 0.01	3.160 ± 0.134	0.229 ± 0.113	31 ± 7	45 ± 4	24 ± 4
Glutamate and malate	0.90 ± 0.06	0.080 ± 0.070	0.09 ± 0.05	0.812 ± 0.141	0.01 ± 0.01	3.343 ± 0.430	0.185 ± 0.132	32 ± 9	43 ± 1	25 ± 8
KCN	0.73 ± 0.05	0.210 ± 0.027	0.21 ± 0.04	0.767 ± 0.110	0.05 ± 0.01	2.465 ± 0.308	0.445 ± 0.030	35 ± 5	36 ± 6	29 ± 4
CCCP	0.73 ± 0.03	0.171 ± 0.038	0.20 ± 0.03	0.849 ± 0.205	0.08 ± 0.03	2.969 ± 0.213	0.512 ± 0.110	24 ± 3	33 ± 6	43 ± 7

Analysis was performed on several mitochondrial ROIs from 20 control cells and four cells under the influence of a drug. The high SDs result from biological variations occurring from measurements on a single-cell or single-mitochondrion level.