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. 2008 Jul 11;95(8):3715–3723. doi: 10.1529/biophysj.108.134064

FIGURE 3.

FIGURE 3

An in silico model of a mutant strain in which CTA1 is solely ORE-activated (AOPY Mutant Model I) is predicted to have greater variability of CTA1 expression than the WT model. The histogram shows the simulated population heterogeneity of reporter expression (CTA1 mRNA level) in WT (black bars) and mutant strain (in which Adr1p does not regulate CTA1) (white bars) in oleate growth conditions. The abscissa is the CTA1 mRNA concentration after 100 min of stochastic simulation with initial conditions given by species concentrations obtained from the steady-state solution to the ODE kinetic model with constant 0.12% (w/v) oleate. Stochastic simulations were carried out for an ensemble of 1000 realizations of the stochastic process. CVmut represents the steady-state CV of reporter expression levels for the AOPY Mutant Model I, and CVWT represents the CV in the WT model.