Figure 4. Impacts of viral proteins provided in trans on the replication/ transcription of SARS-CoV replicon.
(A) Nonstructural proteins; (B) Structural and accessory proteins. 2×105 BHK21 cells were transfected with pBAC- Rep-SCV-luc/neo (0.25 µg), the viral protein expression plasmids (0.2 µg each) and pRL-TK (0.05 µg) as an internal control. The corresponding empty vectors (0.2 µg each) were used as the negative controls. The luciferase activities were measured 24 h post transfection. Error bars represent standard deviations of the mean of three experiments.