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. 2008 Oct 1;3(10):e3299. doi: 10.1371/journal.pone.0003299

Figure 5. The effect of additional N protein on viral genome replication and transcription.

Figure 5

(A) Kinetics of luciferase activity of the reporter replicon. 2×105 BHK21 cells were transfected with pRL-TK plasmid (0.1 µg) and pBAC-Rep-SCV-luc/neo (0.4 µg). After transfection, the cells were collected for luciferase assays at different time points (6 h to 72 h). (B) Reporter gene activity in presence or absence of additional N protein provided in trans. 2×105 BHK21 cells were transfected with pRL-TK plasmid (0.05 µg), pBAC-Rep-SCV-luc/neo (0.25 µg) and pcDNA3.0-N (0.2 µg) or pcDNA3.0 (0.2 µg). After transfection, the cells were harvested for luciferase assays at different time points (6 h to 38 h). (C) The ratios of luciferase activities of Rep-SCV-luc/neo in presence of N protein related to that in absence of N protein at different time points (6 h to 38 h). Error bars represent standard deviations of the mean of three experiments.