Figure 7.

Dyn 3-GFP forms expressed in Clone 9 cells distribute diffusely to the cytoplasm or to unidentified vesicular structures. Fluorescence images of Dyn 3(baa)-GFP-transfected cells reveal a prominent population of brightly labeled vesicles, which did not colocalize with clathrin (a–a") at either the plasma membrane (boxes) or the Golgi apparatus (arrows). These dynamin-associated vesicles did not colocalize with endocytosed dextran (boxed regions), suggesting they are not late endocytic compartments (b–b"). In contrast, cells expressing Dyn 3(aaa)-GFP [a form differing from Dyn 3(baa) by only a 10-amino-acid insertion at the first splicing region] showed only a diffuse cytoplasmic fluorescence with a slight association with the Golgi apparatus, as demonstrated by costaining with an antibody to clathrin (c–c"). Bar, 10 μm.