Fig. 2.
Reverse transcription polymerase chain reaction (RT-PCR) experiments in SCA demonstrated positive expression for all Kir genes examined. The first and last lanes gel are a 100-bp marker, the second and third lanes are negative controls with water being substituted for mRNA and next, no reverse transcription reaction, respectively. For all negative controls, the Kcnj10 primers were used. In each subsequent lane, the primers for the designated gene were used first in SCA (>9 DIC) and then a rat brain cDNA library was used as a positive control. Low detection levels of Kcnj1 in the rat brain prompted us to test a rat kidney (KID) cDNA library as well. The primer pairs and the expected molecular weight of each product are summarized in Table 1.