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. Author manuscript; available in PMC: 2008 Sep 25.
Published in final edited form as: J Immunol. 2003 May 15;170(10):5317–5325. doi: 10.4049/jimmunol.170.10.5317

Table III.

Recognition of DC-TC hybrids by HLA-A*0201-restricted, tumor-reactive T cell populationsa

Expt. Target Cell HLA-A2
Expression		 Cell Description TIL 1200 CK3H6 JR1E2 MB4 JB2F4 GA3D TH1F2L
1 Media − None 254 8 0 33 0 178 14
T2 + HBV:18–26(23Y) + Nonfused 180 2 0 14 20 93 312
T2 + relevant peptide + Nonfused 4,355b >20,000 13,496 >20,000 5,572 6,049 >20,000
888mel − Nonfused 156 0 6 34 8 33 21
526mel CIITA + Nonfused 5,899 4,348 2,493 12,731 7,014 2,546 21
624mel CIITA + Nonfused 5,708 8,918 9,581 >20,000 18,734 9,591 8,592
Donor 2 DC-888melc DC+-mel− Fused adherentd >2,000 3,323 1,553 266 1,577 5,773 68
Donor 2 DC-888melc DC+-mel− Fused nonadherent 328 258 21 63 60 1767 136
Donor 2 DC-888melc DC+-mel− Physically mixed 249 32 2 59 0 516 100
Donor 4 DC-888melc DC+-mel− Fused adherente >2,000 4,148 1,252 190 775 6,050 60
Donor 4 DC-888melc DC+-mel− Fused nonadherent 270 121 13 44 6 2,399 99
888melc − Fused adherent 238 14 18 37 0 22 31
2f Media − None 245 13 8
Donor 2 DC-888melg DC+-mel− Fused adherent 1,407 1,873 253
Donor 5 DC-888melh DC−-mel− Fused adherent 276 16 19
a

IFN-γ secretion (picograms per milliliter) in 20 h coculture supernatants of target cells with T lymphocytes. The data in each of the two panels were generated in separate experiments.

b

Underlined values indicate that IFN-γ secretion in response to peptide-loaded T2 cells, HLA-A*0201+ melanoma cell lines, or hybrid cell populations was 50 pg/ml and at least twice background with any HLA-A*0201− or Ag− target cell (including a physical mixture of 888 melanoma cells and DCs).

c

For these fused cell populations, 1 × 105 T cells were coincubated with 3.75 × 104 target cells.

d

In the adherent fraction of DC-888mel fusion cells from donor 2 in this experiment, 48% of cells were positive for both CFSE and HLA-DR by FACS.

e

In the adherent fraction of DC-888mel fusion cells from donor 4 in this experiment, 29% of cells were positive for both CFSE and HLA-DR by FACS.

f

In the second experiment, 1 × 105 T cells were coincubated with 1.5 × 104 target cells.

g

In the adherent fraction of DC-888mel fusion cells from donor 2 in this experiment, 19% of cells were positive for both CFSE and HLA-DR by FACS.

h

In the adherent fraction of DC-888mel fusion cells from donor 5 in this experiment, 25% of cells were positive for both CFSE and HLA-DR by FACS.