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. Author manuscript; available in PMC: 2008 Sep 25.
Published in final edited form as: J Immunol. 2003 May 15;170(10):5317–5325. doi: 10.4049/jimmunol.170.10.5317

Table IV.

Recognition of DC-TC hybrids by HLA-DRβ1*0401- and -DRβ1*0701-restricted, gp100-reactive T cell populationsa

Expt. Target Cell HLA-DR4/DR7 Expression Cell Description BR-B8 B104
1 Media DR4−DR7− None 11 0
Donor 2 DC-888melb DC++-mel−− Fused adherentc 1,475d 725
Donor 2 DC-888melb DC++-mel−− Fused nonadherent 399 38
Donor 2 DC-888melb DC++-mel−− Physical mixture 0 7
Donor 4 DC-888melb DC++-mel−− Fused adherente 1,475 255
Donor 4 DC-888melb DC++-mel−− Fused nonadherent 243 29
888melb DR4−DR7− Fused adherent 0 0
2f Media DR4−DR7− None 0 1
Donor 2 DC-888melg DC++-mel−− Fused adherent 2,460 68
Donor 5 DC-888melh DC−−-mel−− Fused adherent 8 25
3 Media DR4−DR7− None 0 10
1088 EBV-B + Flu HA:306–324 DR4+DR7− Nonfused 4
1088 EBV-B + gp100:44–59 DR4+DR7− Nonfused 1,664
1978 EBV-B + IgK:188–201 DR4−DR7+ Nonfused 19
1978 EBV-B + gp100:170–190 DR4−DR7+ Nonfused 7,541
888mel DR4−DR7− Nonfused 0 0
F002Rmel CIITA-GFP DR4−DR7+ Nonfused 0 8
F002Rmel CIITA-gp100 DR4−DR7+ Nonfused 10 >20,000
624mel CIITA DR4+DR7+ Nonfused 1,946 9,673
1088mel CIITA DR4+DR7− Nonfused 3,519 25
Donor 2 DC-888melb DC++-mel−− Fused adherentc 5,338 883
Donor 2 DC-888melb DC++-mel−− Fused nonadherent 937 66
Donor 2 DC-888melb DC++-mel−− Physical mixture 31 29
888melb DR4−DR7− Fused adherent 0 0
a

IFN-γ secretion (picograms per milliliter) in 20 h coculture supernatants of target cells with T lymphocytes. The data in each of the three panels were generated in separate experiments.

b

For these fused cell populations, 1 × 105 T cells were coincubated with 3.75 × 104 target cells.

c

In the adherent fraction of DC-888mel fusion cells from donor 2 in this experiment, 48% of cells were positive for both CFSE and HLA-DR by FACS.

d

Underlined values indicate that IFN-γ secretion in response to peptide-loaded EBV-B cells, melanoma cell lines, or hybrid cell populations was 50 pg/ml and at least twice background with relevant control target cells (including a physical mixture of 888 melanoma cells and DCs).

e

In the adherent fraction of DC-888mel fusion cells from donor 4 in this experiment, 29% of cells were positive for both CFSE and HLA-DR by FACS.

f

In the second experiment, 1 × 105 T cells were coincubated with 1.5 × 104 target cells.

g

In the adherent fraction of DC-888mel fusion cells from donor 2 in this experiment, 19% of cells were positive for both CFSE and HLA-DR by FACS.

h

In the adherent fraction of DC-888mel fusion cells from donor 5 in this experiment, 25% of cells were positive for both CFSE and HLA-DR by FACS.