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. Author manuscript; available in PMC: 2008 Sep 25.
Published in final edited form as: Mech Ageing Dev. 2005 Oct;126(10):1090–1096. doi: 10.1016/j.mad.2005.04.002

Fig. 3.

Fig. 3

Elevated ATP levels in ucp-4(ok195) animals. ATP levels in wildtype (filled) and ucp-4(ok195) (open) eggs (a) and L4 larvae (b). The error bars represent S.E.M. from two experiments, and four replicates of each genotype were performed, (c) JC-1 staining was performed to compare mitochondrial membrane potential of wildtype and ucp-4(ok195) animals. JC-1 mitochondrial accumulation is mitochondrial membrane potential dependent, and greater JC-1 concentrations promote dye aggregation, shifting fluorescence from green to red. The red:green ratio of mitochondrial JC-1 fluorescence reflects mitochondrial membrane potential and tended to be slightly greater in ucp-4(ok195) animals than in wildtype animals, although this difference was only statistically significant at 0.66 μg/mL (p = 0.01, t-test).