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. Author manuscript; available in PMC: 2008 Sep 25.
Published in final edited form as: Biochemistry. 2007 May 16;46(24):7284–7292. doi: 10.1021/bi700077v

Figure 3. Changes to the absorption spectra of cob(III)alamin after the photolysis of adenosylcobalamin and from the addition of 5′-peroxyadenosine.

Figure 3

(A) Photolyzed adenosylcobalamin (80 µM) held for 20 h at pH 8.5. The initial spectrum decays to that of cob(III)alamin (- - -). Inset: Biphasic decay of reaction intermediate monitored spectrophotometrically at 312 nm. (B) Cob(III)alamin in the presence (80 µM) of 400 µM 5′-peroxyadenosine and held 25 min at pH 8.5. The initial absorption spectrum of 80 µM cob(III)alamin at pH 8.5 is ( - - -).