Table 3. Analysis of AEF activity in peripheral blood monocytes isolated from mice with AA amyloidosis.
Donor mice | Recipient mice | |||
Mouse | Amyloid grade | Group | No. of mice with amyloid/total no. of mice | Amyloid grade |
G1 | 4+ | H1 | 5/7 | 1+–2+ |
G2 | 4+ | H2 | 5/5 | 1+–3+ |
G3 | 4+ | H3 | 4/8 | 1+–2+ |
G4 | 4+ | H4 | 1/6 | 1+ |
G5 | 4+ | H5 | 0/6 | − |
G6 | 4+ | H6 | 2/6 | 1+–2+ |
G7 | 4+ | H7 | 0/5 | − |
G8 | 4+ | H8 | 2/5 | 1+–2+ |
The table presents detailed information on animals in group H 1–8. AA-amyloidosis was induced by i.v. injection of AEF and 0.2 ml 1% silver nitrate injections on day 1, 7, 14, 21 and 28. The animals were sacrificed on day 35. Blood was collected and amyloid was verified in spleen sections after Congo red staining. Isolated peripheral blood monocytes were injected into new animals, group H 1–8, and silver nitrate was given day 1, 7 and 14.The animals were sacrificed day 16 and the presence of amyloid was studied in spleen after Congo red staining.