Table 4. Analysis of AEF activity in peripheral blood monocytes isolated from mice with AA amyloid induced by monocytes isolated from mice with AEF-induced AA amyloid.
Donor mice | Recipient mice | |||
Mouse | Amyloid grade | Group | No. of mice with amyloid/total no. of mice | Amyloid grade |
H2-1 | 1+ | J1 | 2/3 | 1+–2+ |
H2-2 | 3+ | J2 | 3/3 | 1+ |
H2-3 | 2+ | J3 | 2/3 | 1+ |
H2-4 | 1+ | J4 | 3/3 | 1+–2+ |
H2-5 | 2+ | J5 | 2/3 | 2+ |
Mice in group J received an i.v. injection of monocytes isolated from H2 (1–5) and a sequential s.c. injection of 0.2 ml 1% silver nitrate day 1, 7 and 14 and were sacrificed day 16.The presence of amyloid was analyzed in spleen after Congo red staining.