Table 5. Analysis of AEF activity in monocytes isolated at different time points after AEF and AgNO₃ injection.

Treatment of mice prior to monocytes isolation						Recipient mice
Group	AEF	AgNO3	Duration	No. of mice with amyloid/total no. of mice	Amyloid grade	Group	No. of mice with amyloid/total no. of mice	Amyloid grade
K1	+	+	2 min	0/3	−	L1	0/9	−
K2	+	+	6 hours	0/3	−	L2	0/9	−
K3	+	+	48 hours	3/3	1+	L3	0/9	−
K4	+	+	7 days	3/3	2+	L4	5/9	1+

Mice in group K received an i.v. injection of 0.1 ml AEF with a sequential s.c. injection of 0.2 ml 1% silver nitrate and were sacrificed 2 minutes, 6 and 48 hours, and 7 days later. Peripheral blood monocytes were isolated, sonicated and injected in to new groups with three animals in each, group L. These mice received inflammatory stimuli day 1, 7 and 14 and were sacrificed day 16. The presence of amyloid was analyzed in spleen after Congo red staining.