Skip to main content
. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Cancer Res. 2008 Jul 1;68(13):5309–5317. doi: 10.1158/0008-5472.CAN-08-0504

Fig. 5.

Fig. 5

MT-hTer-47A expression induces telomere elongation in ATM-depleted UM-UC-3 cells. UM-UC-3 cells were infected with lentivirus expressing scramble #1 and ATM #2 shRNAs at day -2, followed by lentivirus expressing WT-hTER and MT-hTer-47A at day 0. Cells were harvested at day 8 and genomic DNA was isolated and digested with HinfI and RsaI. A. Southern blotting was performed using an oligonucleotide probe for wild-type telomeric DNA (5′-(CCCTAA)4-3′). Adjacent graphs show signal intensities for each lane, and arrows indicate the approximate peak signal in each. B. Southern blotting was performed with an oligonucleotide probe targeting the mutant telomeric DNA (5′-(CCCAAA)4-3′) (left panel). A four-fold excess of two unlabeled oligonucleotides (5′-(CCCTAA)4-3′ and 5′-(CCCCAA)4-3′) was added to the mutant probe hybridization to block nonspecific binding to wild-type and subtelomeric sequences. The blot was then stripped and hybridized to the wild-type telomeric probe (right panel).