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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Cancer Res. 2008 Jul 1;68(13):5309–5317. doi: 10.1158/0008-5472.CAN-08-0504

Fig. 6.

Fig. 6

MT-hTer-47A expression induces telomere fusions in an ATM-dependent manner. LOX cells were infected with lentivirus expressing scramble #1 and ATM #2 shRNAs at day -2, followed by lentivirus expressing WT-hTER and MT-hTer-47A at day 0. Cells were treated with colcemid on day 3, and metaphase spreads were prepared. Telomeres were labeled by fluorescence in situ hybridization. A. A representative image showing multiple telomere fusions (arrows) in a cell treated with scramble #1 shRNA and MT-hTer-47A. B. A histogram showing the percentage of metaphase spreads containing different numbers of telomeric fusions. Between 22 and 37 metaphase spreads were analyzed for each sample type, and results were consistent across two independent experiments. In scramble #1-treated cells, MT-hTer-47A caused a significant increase in fusions compared to WT-hTER (p = 0.0001). In MT-hTer-47A-treated cells, ATM knockdown significantly reduced the number of fusions observed compared to the scramble #1 control (p < 0.0001).