(a) Only the combination of TN-C (CEF-TN) and bFGF promote an elongated phenotype in nonsprouting BAECs. Tissue culture dishes were coated with CEF-TN (a, b) or hFN (c, d) or left uncoated (e, f). Nonsprouting BAECs were plated in the presence or absence of bFGF. In the presence of bFGF (b, d, f), a strong elongation of the cells was found on CEF-TN (b, arrows) but not on hFN (d) or plastic (f). No phenotypic change was found in the absence of bFGF on either substrate (a, c, e). Photos were taken after 48 h of incubation. Bars, 75 μm. (b) Quantification of elongated phenotypes in nonsprouting BAECs plated on CEF-TN, hFN, or plastic in the presence or absence of bFGF. Data represent the percentage of elongated cells from the total cell population. Mean ± SD from one representative experiment is shown (n = 3). Statistical analysis: significant (*), not significant (n.s.); P < 0.05.