Fig. 1.

(A) Elution profiles of Superdex 200 HR gel filtration chromatography. The column was eluted with Tris-HCl buffer (0.01 M, pH 7.4) containing 0.1 M NaCl at a flow rate of 0.3 ml/min. Cysteine protease inhibitor activity (CPI) fractions are indicated as bar. The active fractions were pooled and concentrated. (B) Elution profiles of 2nd Resource Q anion exchanger chromatography. The column was eluted with Tris-HCl buffer (0.01 M, pH 7.4) increasing molarity of NaCl. The flow rate was 1 ml/min, and it was collected 0.5 ml/tube. The CPI fractions are indicated as bars.