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Gelatin containing SDS-PAGE findings of purified cysteine protease inhibitor (CPI) under non-reducing condition. The gel was analyzed on 7.5-15% gradient gel. M, standard marker proteins; lane 1, partially purified 27 kDa cysteine protease (CP) without inhibitor; lane 2, 27 kDa CP with CPI (5 µg); lane 3, 27 kDa CP with CPI (10 µg); lane 4, 27 kDa CP with cystatin (10 µg); lane 5, 27 kDa CP with iodoacetic acid (IAA, 20 µM). Proteolysis was shown on white area.
