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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2008 Aug;7(8):2556–2565. doi: 10.1158/1535-7163.MCT-08-0015

Figure 5.

Figure 5

FASN inhibition is associated with a drop in ChoK activity (A) but not in long term inhibition of PI3K singling (B). A. 1H MR spectrum of cell lysates showing endogenous PCho and added Cho. Inset: representative plot of PCho synthesized by ChoK extracted from control cells and 48 h Orlistat-treated cells. Lines represent the straight-line fits to the data used to obtain rates of PCho synthesis (R2 = 0.89, Control; R2 = 0.96, Orlistat) indicating inhibition of ChoK following Orlistat treatment. B. Western blot analysis of P-Akt, Akt and GAPDH (loading control) expression in control and treated PC-3 cells following 3, 24 and 48 hours of Orlistat treatment.