Table 5.
Femoral static and dynamic bone histomorphometry of 1-month-old CTGF transgenics and wild-type control females, line 1
| Line 1 | WT | CTGF+/+ |
|---|---|---|
| Bone volume/tissue volume (%) | 13.2 ± 1.1 | 9.6 ± 0.9a |
| Trabecular separation (μm) | 85 ± 7 | 116 ± 6a |
| Trabecular number (mm−1) | 10.6 ± 0.7 | 7.9 ± 0.4a |
| Osteoblasts/bone perimeter (mm−1) | 46.9 ± 3.6 | 48.7 ± 2.3 |
| Osteoblasts/tissue area (mm−2) | 766 ± 52 | 605 ± 38a |
| Osteoclasts/bone perimeter (mm−1) | 10.6 ± 0.5 | 10.5 ± 0.8 |
| Eroded surface/bone surface (%) | 25.3 ± 1.5 | 24.1 ± 1.3 |
| Mineralizing surface/bone surface (%) | 3.4 ± 0.7 | 2.1 ± 0.6 |
| Mineral apposition rate (μm/d) | 1.10 ± 0.07 | 1.11 ± 0.05 |
| Bone formation rate/bone surface (μm3/μm2/d) | 0.040 ± 0.011 | 0.023 ± 0.006 |
Bone histomorphometry was performed on femurs from 1-month- old female CTGF homozygous (CTGF+/+), and wild-type (WT) controls for line 1. For static histomorphometry, sections were stained with toluidine blue, and for dynamic histomorphometry unstained sections were analyzed by fluorescence microscopy. Values are means ± sem; n = 6 to 9, except for dynamic histomorphometry, where n = 4 to 6.
a
Significantly different from wild-type controls, P < 0.05.