Table 7.
Expression of osteoblast gene markers in stromal cells from CTGF transgenics and wild-type controls
| Confluence
|
10 d after confluence
|
|||
|---|---|---|---|---|
| Stromal cells | WT | CTGF | WT | CTGF |
| Alkaline phosphatase | 6.3 ± 1.7 | 2.8 ± 0.1 | 8.6 ± 1.0 | 4.0 ± 0.4a |
| Osteocalcin | 2.1 ± 0.4 | 1.9 ± 0.1 | 21.0 ± 3.0 | 7.7 ± 1.4a |
| Bsp | 2.7 ± 0.4 | 0.9 ± 0.4a | 3.7 ± 1.6 | 2.1 ± 0.8 |
| Ctgf | 1.3 ± 0.1 | 2.1 ± 0.1a | 0.8 ± 0.1 | 1.8 ± 0.1a |
Bone marrow stromal cells from CTGF heterozygous transgenics (line 2) and wild type (WT) controls were cultured for 10 d after confluence. Total RNA was reverse-transcribed, and amplified by real-time RT-PCR in the presence of specific primers. Data are expressed as alkaline phosphatase, osteocalcin, bsp and ctgf copy number, determined by real-time RT-PCR, corrected for rpl38 expression. Values are means ± sem, n = 4.
a
Significantly different from wild-type controls, P < 0.05.