Figure 7.

Effect of ACTH on steroidogenic gene expression and cortisol production. Interrenal tissue slices were incubated with either control (no ACTH) or varying concentrations of ACTH for 8 h and medium cortisol levels (A), and tissue mRNA levels of MC2R (B), StAR (C), and P450scc (D) determined by qPCR. Values are shown as percent control and represent mean ± sem (n = 4–5 fish); different letters denote statistically significant differences between different ACTH concentrations (repeated measures ANOVA, followed by Bonferonni post hoc test; P < 0.05).