Figure 8.

Effect of α-MSH on in vitro cortisol production (A) and MC2R levels (B) in rainbow trout interrenal tissue preparations. Interrenal tissue slices were incubated with either control (no α-MSH) or α-MSH (10⁻⁶ to 10⁻¹⁰ m) for 8 h, and both medium and tissue slices were sampled for determining cortisol production and MC2R mRNA levels, respectively. Values are shown as percent unstimulated control and represent mean ± sem (n = 4–5 fish); no significant differences were observed between different MSH concentrations (repeated measures ANOVA, followed by Bonferonni post hoc test; P < 0.05).