Table 1.

List of primer sequences used to obtain full-length MC2R cDNA as well as mRNA abundance using qPCR

Primer	Sequence (5′–3′)	Accession no.	Product size (bp)
MC2R forward	CACCATCTTCCACGCACTGC
MC2R reverse	CCTGGTGTGGGATCGAGCC		225
5′-RACE	AGCCAGCAGGAACATGTGGACGTA		649
3′-RACE	TCCACGCCCTGCGCTACCAC
3′-Nested	CGGGGATCTGGGCGCTATGC		836
Full MC2R forward	TGTGGACGTTGCTCTGACAC
Full MC2R reverse	GGGTCAAAAACTCTGGTGGA
MC2R forward (qPCR)	GAGAACCTGTTGGTGGTGGT	EU119870	105
MC2R reverse (qPCR)	GAGGGAGGAGATGGTGTTGA
StAR forward	TGGGGAAGGTGTTTAAGCTG	AB047032	101
StAR reverse	AGGGTTCCAGTCTCCCATCT
P450scc forward	GCTTCATCCAGTTGCAGTCA	S57305.1	140
P450scc reverse	CAGGTCTGGGGAACACATCT
β-Actin forward	TGTCCCTGTATGCCTCTGGT	AF157514	121
β-Actin reverse	AAGTCCAGACGGAGGATGG