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Binding of E2-BSA-FITC to P6 hamster ovarian cells. Cells were mechanically dispersed from ovaries, washed, and immediately exposed to E2-BSA-FITC (A), E2-BSA-FITC + E2-BSA (B), or E2-BSA-FITC + E2 (C) as indicated in the experimental design. Bar, 10 μm. D, FITC signal was digitized and expressed as OD per pixel. Both E2-BSA and E2 significantly attenuated E2-BSA-FITC binding to ovarian cells. Con, Control. Bars with different letter, P < 0.05; bars with same letter, P > 0.05.
