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. 2008 May 22;149(9):4452–4461. doi: 10.1210/en.2008-0441

Figure 6.

Figure 6

siRNA knockdown of GPR30 protein and E2-BSA-FITC binding in P6 ovarian cells cultured for 96 h. A, Binding of E2-BSA-FITC to P6 ovarian cells transfected with nontargetting siControl RNA. B, DIC image of cells in A. C, Perinuclear E2-BSA-FITC binding to MCF7 breast cancer cells. D, Western blot showing GPR30 protein levels in cells exposed to siControl or GPR30 siRNA. The top panel shows a blot of two samples each of control and GPR30 siRNA-treated cells probed with the GPR30 antibody, the middle panel shows the levels of tubulin, and the bottom panel shows the OD of GPR30 protein relative to tubulin. Con, Control. Each bar represented a mean OD ± sem of three samples. Bars with different letter, P < 0.05. E, Binding of E2-BSA-FITC to P6 ovarian cells transfected with GPR30 siRNA. F, DIC image of the cell in E. Bar, 10 μm.