Splenic dendritic cell (DC) reconstitution by untreated bone marrow (BM) precursors is unchanged by ethanol (EtOH) feeding. (A) Representative gating strategy used for identifying donor-derived DC subsets following BM reconstitution. CD45.1+ (donor-derived) cells were gated, and within that population CD11c+ cells (DC) that were also positive for each of the subset markers (B220, shown; CD8, CD4, or CD11b) were gated. (B) Reconstitution of 4-week- and (C) 16-week-EtOH-treated recipients and controls. Weeks on EtOH represent length of feeding at the time of BM transfer; weeks of reconstitution represent additional time on the same (EtOH or water) regimen after BM transfer. For each exposure period, the upper left graph shows the total number of donor-derived splenocytes at each time-point examined. The lower left graph represents total donor-derived splenic DC numbers, which were then subdivided into 4 DC subsets (center and right columns of graphs). Time-points with error bars had ≥3 mice/group. The 2 time-points without error bars represent 2 mice/group. Error bars = SD. *p ≤ 0.05 for water vs. EtOH.