Figure 2.
The first 26 amino acids of Hmg1p stabilized Hmg2p. (A) Cycloheximide-chase assay of strains expressing either wild-type Hmg2p or Hmg2p with the first 26 amino acids replaced with those from Hmg1p (2–11–26). Cycloheximide was added to cultures at 0 h, and samples were lysed immediately or after a 4-h degradation period and then analyzed by immunoblotting with the 9E10 anti-myc antibody. (B) Effect of ZA on the log-phase, steady-state levels of normal Hmg2p or 2–11–26. Cultures of strains expressing the normal or chimeric protein were allowed to grow in the absence (−) or presence (ZA) of 25 μg/ml ZA. Cell samples were then lysed and immunoblotted as in panel a. (C) Amino acid sequence comparison of the first 26 amino acids of Hmg1p and Hmg2p. In all figures, the strain used to express a given recombinant or optical reporter can be found in Table 1.