Figure 5.

Effect of lovastatin on 2–1_27–24 was less potent than on normal Hmg2p. (A) Effect of drug treatments on the log-phase, steady-state levels of normal Hmg2p or 2–1_27–54. Log-phase cultures of strains expressing the indicated protein were grown in the presence of the indicated doses of lovastatin (Lov), or 25 μg/ml ZA. Levels were determined by immunoblotting with the 9E10 anti-myc antibody after a 4-h growth period. (B) Effect of indicated doses of lovastatin on the log-phase, steady-state levels of Hmg2p-GFP or 2–1_27–54-GFP in strains with identical HMG-R activity. The strains expressing each reporter protein were otherwise isogenic, and each expressed the soluble, nondegraded Hmg2p catalytic domain as the sole source of HMG-R activity. Each strain was grown in the indicated dose of lovastatin (μg/ml) for 3 h and then analyzed by FACS for levels of the fluorescent reporter protein. Horizontal axis represents arbitrary fluorescence units on a log scale. Vertical axis represents cell numbers for a given fluorescence intensity. A total of 10,000 cells for each histogram were analyzed.