Figure 8.
The first 26 amino acids of Hmg1p stabilized the poorly regulated 2–127–54. (A) Cycloheximide-chase assays of strains expressing normal Hmg2p, 2–127–54, or 2–11–54 (which has both the stabilizing and the deregulating replacements). After addition of cycloheximide, lysates were prepared at the indicated times and immunoblotted with the 9E10 anti-myc antibody. (B) The log-phase, steady-state levels of normal Hmg2p, 2–127–54, or 2–11–54 were assayed in the same strains as in panel a but grown in the presence of either no drug (−), or 25 μg/ml ZA. The levels of Hmg2p in each strain were assayed by immunoblotting with the 9E10 anti-myc antibody after a 3-h growth period in the drugs.