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. 2008 Sep 8;8:142. doi: 10.1186/1471-2180-8-142

Figure 1.

Figure 1

Haemagglutination by E. coli or Salmonella expressing PagN. (A) Cultures of E. coli strain XL-1 Blue harboring plasmids pML1 (PagN+) or pTrc99a (PagN-) were induced with IPTG and then mixed with 3% human blood. Overnight cultures of E. coli harboring plasmids pHek6 (Hek+) and pBSKII+ (Hek-) were included as a positive control and vector control respectively. (B) Assays were carried out as described in Methods with cultures being incubated at 37°C or 70°C for 30 min prior to the assay. (C) The effect of LPS on PagN-promoted agglutination. S. Typhimurium strain LT-2 or CH133 harboring either pBSKII+ or pPagN2.3 were grown in MOPS minimal media adjusted to pH 5.8. Haemagglutination assays were performed as described in Methods.