Table 2.
Bacterial plasmids used in this work
| Plasmid | Relevant Features | Reference |
| pACYC184 | Cmr Tetr General cloning vector | [35] |
| pBSKII+ | Apr ColE1 replicon | Stratagene |
| pBR322 | Apr Cloning vector | [36] |
| pHek6 | Apr hek gene in Pbskii+ | [14] |
| pHP45Ω | Apr Spcr pMB1 replicon | [37] |
| PInvA | Apr invA gene with 500 bp flanking DNA cloned into pBSKII+ | This study |
| pInvAKO | Apr invA::cat gene with 500 bp flanking DNA cloned into pBSKII+ | This study |
| pKOBEGA | Apr Temperature-sensitive plasmid carrying the red and gam genes of λ-phage under control ParaBAD promoter | [32] |
| pMALc2 | Apr MBP fusion vector | New England Biolabs |
| pML1 | Apr pagN ORF inserted into pTrc99a | This study |
| pML4 | Apr pagN ORF flanked by NcoI and BamHI sites inserted into pBSKII+ | This study |
| pML7 | Apr pagN ORF, excluding the DNA corresponding to the signal sequence of the PagN protein, inserted into pMAL-c2 | This study |
| pML10 | Apr pagN gene inserted into pBR322 | This study |
| pPagN2.3 | Apr pagN gene with 500 bp flanking DNA cloned into pBSKII+ | This study |
| pPagNKO | Apr Spcr pagN::spc | This study |
| pTrc99a | Apr Cloning vector with an IPTG-inducible Ptrc promoter | [38] |