Figure 1.

Transiently transfected hippocampal neurons express the full-length human Staufen-GFP fusion protein. Western blots of extracts from either transiently transfected (S) or mock-treated (M) rat hippocampal neurons were probed with a polyclonal GFP antibody. The antibody detects a 92-kDa band in the transfected but not in the untransfected neurons; this represents the expected size for the hStau-GFP fusion protein (65 + 27 kDa). Additionally, the antibody detects a 61-kDa band in all neuronal extracts, most likely representing an unspecific cross-reaction. The sizes of the molecular mass markers are indicated on the left in kilodaltons.