Figure 3.

Human Staufen-GFP positive granules colocalize with RNA in transiently transfected rat hippocampal neurons. RNA was visualized using SYTO14 (Knowles et al., 1996; Kiebler et al., 1999), and the degree of colocalization with hStau-GFP was revealed by fluorescent microscopy (see MATERIALS AND METHODS). Individual green fluorescent hStau-GFP granules (A) were analyzed for the presence of RNA detected by SYTO14 labeling of the same cell (B). Arrowheads indicate hStau-GFP granules containing RNA. Overall, 65.6% of analyzed hStau-GFP granules were RNA positive. The additional SYTO14 labeling in B might reflect both RNA-positive granules containing endogenous Staufen as well as mitochondria (Knowles et al., 1996).