Table IV. Selected proteins related to R. leguminosarum LpxE.
Possible orthologs in the NCBI database as of May 30, 2003 were identified with the PSI-Blast algorithm (86), using the predicted R. leguminosarum LpxE protein sequence of 244 residues as the probe. The low complexity filter was removed. The Legionella and Franciscella orthologs were found using the tBLASTn algorithm at www.ncbi.nlm.nih.gov/BLAST/ or artedi.ebc.uu.se/Projects/Francisella/blast/, respectively. A putative protein identical to our LpxE sequence is also predicted by tBLASTn searching of the R. leguminosarum genome at www.sanger.ac.uk/Projects/Microbes/.
| Organism | Homology (Gaps)a | Approximate E values |
|---|---|---|
| A. tumefaciensb | 84/125/214 (4) | 5 × 10− 30 |
| S. melilotic | 87/123/222 (2) | 6 × 10− 26 |
| M. loti | 83/108/223 | 8 × 10− 26 |
| F. tularensis | 51/65/112 (1) | 1 × 10− 21 |
| Legionella pneumophila | 55/72/149 | 3 × 10− 16 |
| Magnetospirillum magnetotacticum | 69/101/209 (18) | 4 × 10− 14 |
| Brucella melitensis 16M | 40/61/119 | 4 × 10− 11 |
Homology is given as the number of identities/number of positives/number of residues (including gaps) in the related segment when compare with R. leguminosarum LpxE, a hypothetical protein of 244 amino acid residues.
There are two separately sequenced A. tumefaciens strains. This is the strain sequenced by the University of Washington (88). The strain sequenced by the Cereon group contains the same ortholog (87).
This organism contains an LpxE ortholog and a cluster of genes similar to that present in R. leguminosarum 3841.