Table 2.

Concentration dependent inhibition of [³H] biotin uptake in MDCK-MDR1 cells by unlabelled biotin, pantothenic acid and desthiolbiotin

Inhibitors	Concentration (μM)	Uptake as of control	P
Biotin	0	100 ± 4.85
	10	51.50 ± 7.46	< 0.01
	100	16.27 ± 0.47	< 0.01
pantothenic acid	0	100 ± 5.02
	10	27.22 ± 1.06	< 0.01
	100	25.62 ± 2.06	< 0.01
desthiolbiotin	0	100 ± 3.27
	10	40.97 ± 4.29	< 0.01
	100	16.67 ± 1.69	< 0.01

Uptake of [³H] biotin (10 nM) was measured in DPBS buffer (pH 7.4) at 37 °C for 5 min in the presence of various concentrations of unlabelled biotin, pantothenic acid and desthiolbiotin. Data are shown as mean ± SD, n = 4–6.