Table 1.

Basic amino acids identified as candidate mediators of IP₆-binding by Ku—Ku70 sequence alignment

Human	Mouse	Rat	Hamster	Chicken	Xenopus	Zebrafish	S. pombe	S. cerevisiae
K 357	K	K	K	K	K	K	K	H
K 358	K	N	K	Q	K	L	P	Y
K 443	K	K	K	K	I	I	I	I
R 444	R	R	R	R	R	R	R	R
K 445	K	K	K	N	K	T	S	K

Accession numbers for the Ku70 amino acid sequences—Human, AAH12154; mouse, BAA28874; rat, AAH78718; hamster, AAB46854; chicken, BAA32018; Xenopus, NP_001082274; zebrafish, ABI54461; S. pombe, O94395; S. cerevisiae, P32807. Multiple sequence alignment was done using Clustal W (1.83). Amino acids that may participate in IP₆ binding by human Ku and the corresponding residues of other species as shown. Basic amino acids (lysines, K or arginines, R) are in bold. Lysine residues mutated in this study are underlined.