Table 3.

Calculated thermodynamics of unfolding of a B-DNA duplex in closed DNA

fGC	[Na]/M	Tm/K	ΔH/ kcal mol−1 bp	ΔG(293) kcal mol−1	ΔG(310) kcal mol−1
0.2	0.075	345.9	8.0	29.3	19.9
0.5	0.075	358.4	8.5	37.3	27.6
0.8	0.075	371	9.1	45.7	35.7
0.2	0.11	349	8	30.8	21.4
0.5	0.11	361.2	8.5	38.6	29
0.8	0.11	373.4	9.1	46.9	36.9
0.2	0.2	353.9	8	33	23.8
0.5	0.2	365.6	8.5	40.6	31.1
0.8	0.2	377.3	9.1	48.6	38.8

Loop size = 24 nt, i.e. for a 22-mer + 1 base at either end.